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Durastab Medtec LTD has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Until now, Durastab has provided products or services to more than 600 companies and 500 scientific research institutes, of which more than 50 IVD companies are listed companies in China or overseas.
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Flagship Product: Safer, More Stable, and More Versatile – ProCide™ 300 Preservative!
Newly Upgraded 3rd-Gen TMB Substrate: Enhanced Stability with Multiple Freeze-Thaw Tolerance!
New Product Launch – Bovine Serum Albumin (BSA) for Clinical Biochemistry, Immunoassays, and Molecular Detection!
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AP Blotting ECL Substrate AMPPD
$0
AP Blotting AMPPD ECL is an ultra‑sensitive chemiluminescent substrate for alkaline phosphatase (AP), containing AMPPD and an enhancer. It is designed for direct use on nitrocellulose (NC) membranes in protein or nucleic acid blotting assays. Combining low background emission with high‑intensity light output, this product enables detection of AP conjugates with maximum sensitivity and signal‑to‑noise ratio.
AP Blotting ECL Substrate AMPPD
AP Blotting AMPPD ECL is an ultra‑sensitive chemiluminescent substrate for alkaline phosphatase (AP), containing AMPPD and an enhancer. It is designed for direct use on nitrocellulose (NC) membranes in protein or nucleic acid blotting assays. Upon mixing with AP, the substrate decomposes immediately and releases photons, providing an extended luminescence plateau. Combining low background emission with high‑intensity light output, this product enables detection of AP conjugates with maximum sensitivity and signal‑to‑noise ratio.
Art no: CLBM
Package size: 100mL, 1L, 5L, bulk
Shelf life:1.5 years
Storage: 2-8℃ protected from light.
Application: Western blot / Northern blot
Instruction:
For a Western blot with a membrane area of 100 cm²:
1.Membrane Blocking: After transfer, briefly rinse the membrane with PBS or TBS, then incubate in 10 mL blocking solution for 30–60 minutes.
Note: PBST or TBST can be used for washing NC or PVDF membranes. For nylon membranes, TBST may yield slightly higher background than PBST.
2. Primary Antibody Incubation: Dilute the primary antibody in 5 mL blocking solution to the manufacturer‑recommended concentration and incubate for 30–60 minutes.
3. Washing: Wash NC and nylon membranes in 20 mL washing buffer (PBST/TBST) for at least 5 minutes per wash, repeated 3 times.
4.Secondary Antibody Incubation: Dilute the AP‑conjugated secondary antibody 1:50,000 in 5 mL blocking solution and incubate for 30–60 minutes.
5.Washing: Repeat the wash as in step 3 (3 × 5 minutes), then wash the membrane twice for 2 minutes each with 20 mM Tris (containing 1 mM MgCl₂, pH 9.8).
6.Removal of Wash Buffer: Drain the wash buffer from the membrane, taking care not to let the membrane dry out.
7.Substrate Incubation: Apply 3 mL of AMPPD substrate solution to the surface of the membrane and incubate for 5 minutes.
8.Removal of Substrate: Drain the substrate solution and place the membrane in an imaging cassette or wrap it in plastic, carefully removing any air bubbles or wrinkles.
9.Membrane Imaging: Initial exposure is recommended for 5–30 minutes. Exposure times for PVDF or nylon membranes are generally shorter than for NC membranes.
For research use only or for further use in the production of detection kits. Not for human or animal use!
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