Durastab Medtech Co., Ltd. has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
About Us
Search
Search
APS-5 is an ultrasensitive chemiluminescent substrate for Alkaline Phosphatase (AP) enzyme-induced reactions. Upon catalysis by AP, it rapidly emits photons, reaching a plateau phase within seconds. Within a specific range of AP enzyme concentration, the emitted photon count is directly proportional to the enzyme concentration in solution.
Disodium (4-Chlorophenylmercapto) (10-Methyl-9, 10-Dihydroacridine Methylene) Phosphate
Disodium (4-Chlorophenylmercapto) (10-Methyl-9, 10-Dihydroacridine Methylene) Phosphate
APS-5 is an enzyme-induced chemiluminescent substrate utilizing Alkaline Phosphatase (AP) as the label. Catalyzed by AP, APS-5 rapidly releases photons, achieving a signal plateau within seconds. The emitted light intensity is proportional to the AP enzyme concentration within a defined range, making it ideal for chemiluminescence immunoassay (CLIA) systems employing AP as the label.
Art no: APPO
Package size: 1g/ 10g/ 50g/ 100g/ 200g
CAS: 193884-53-6
mol wt: 489.817
Purity: ＞99%
Shelf life: 2 year
Storage: -20~-15℃, sealed, dry and protected from ligh.
Application: CLIA、ELISA
Intended Use:
For immunoassays utilizing Alkaline Phosphatase (ALP) as the label.
Important Notes:
1. After preparation into the APS-5 working solution, allow the reagent to equilibrate to room temperature (22–35°C) prior to use.
2. Avoid exposure to strong light under all circumstances.
3. Avoid freezing the prepared liquid substrate solution.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Store at -20 to -15 °C protected from light.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Q1: Which enzyme are AMPPD and APS-5 used with?
A:Both are chemiluminescent substrates for alkaline phosphatase (AP) in CLIA and other luminescent immunoassays, but they belong to different chemical classes:
AMPPD is a 1,2-dioxetane (adamantyl dioxetane) substrate that produces a sustained glow-type signal, typically reaching plateau after minutes of incubation.
APS-5 is an acridan-based (9,10-dihydroacridine) substrate. Upon AP-catalyzed dephosphorylation, it generates high-intensity luminescence that reaches peak signal within seconds.
Thanks to its rapid kinetics, APS-5 is well suited for time-critical applications such as emergency testing, POCT, and high-throughput automated platforms.
Q2: Should I choose APS-5 or AMPPD?
A: APS-5 delivers fast peak light emission with high intensity, which suits rapid automated CLIA platforms; AMPPD produces a longer-lasting glow signal that is economical for high-throughput routine testing.
Q3: Powder or ready-to-use solution?
A: The CAS-grade powders (AMPPD, APS-5) are for self-formulation, while CLAM (AMPPD solution) and CLAP (APS-5 solution) are ready-to-use formulations that remove preparation variability.
Q4: How should the substrates be stored?
A: Ready-to-use solutions: 2-8 °C protected from light. CAS-grade powders: -20 °C protected from light (APS-5: -20 to -15 °C), aliquoted to avoid repeated freeze-thaw of stock solutions.
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send RequestDurastab Medtech (Durastab IVD) is an IVD raw materials manufacturer based in Shenzhen, China, supplying ELISA and CLIA substrates, enzyme stabilizers, blocking buffers and reagent preservatives to in vitro diagnostic manufacturers worldwide.
By submitting this form,you consent to receive marketing updates from Durastab Medtech (Durastab IVD) .
