Office Location
Shenzhen, China
Production Address
B1501, Meixun Science Park,
No.19, Jinxiu Zhonglu ,
Pingshan.
Durastab Medtec LTD has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Until now, Durastab has provided products or services to more than 600 companies and 500 scientific research institutes, of which more than 50 IVD companies are listed companies in China or overseas.
About UsBCIP/NBT purple Fa One Component Membrane Substrate provides a deep purple precipitate upon reaction with ALP. It is the fastest-reacting and most intensely colored BCIP/NBT reagent in the series, suitable for reaction times of approximately 10 minutes. It is used in Western blot, Northern blot, Microarray, ELISpot, and other applications.
Art no: BCFA
Package size: 100mL, 1L, 5L, 10L, bulk
pH value: 9.8±0.3
Shelf life: 3 years
Storage: 2-8℃
Application: Western blot, Microarray, ELISpot
BCIP/NBT purple Fast One Component Membrane Substrate
Durastab™ Series BCIP / NBT membrane / precipitated substrates have low background, stability, sensitivity, and small batch to batch variation. The series substrates provide blue, purple signal systems while providing dedicated immunohistochemical BCIP / NBT substrates.
BCIP/NBT purple Fast One Component Membrane Substrate provides a deep purple precipitate upon reaction with ALP. It is the fastest-reacting and most intensely colored BCIP/NBT reagent in the series, suitable for reaction times of approximately 10 minutes. It is used in Western blot, Northern blot, Microarray, ELISpot, and other applications.
Cat no: BCFA
Package size: 100mL, 1L, 5L, 10L, bulk
pH value: 9.8±0.3
Shelf life: 3 years
Storage: 2-8℃
Application:
Western blot、Northern blot、Microarray、ELISpot etc.
Instruction:
Durastab™ BCIP/NBT purple Fast One Component Membrane Substrate is a ready-to-use type substrate and used after equilibration to the reaction temperature.
Western blot coloration steps:
1. Wash: After incubation with AP, wash with TBST washing buffer for 3 times;
2. Coloration:Add 1mL substrate, incubate for a certain time at 20~37℃, detect strip color is dark purple;
3. Stop: Add 1.5mL deionized water for 2-3 times before background color appears;
4. Read: Visual interpretation or instrument photography to analyze the gray values.
For research use or further use in the production of diagnostic kits only. Not for direct use on humans or animals!

Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send Request B1501, Meixun Science Park,
No.19, Jinxiu Zhonglu ,
Pingshan.

