Office Location
Shenzhen, China
Production Address
B1501, Meixun Science Park,
No.19, Jinxiu Zhonglu ,
Pingshan.
Durastab Medtec LTD has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Until now, Durastab has provided products or services to more than 600 companies and 500 scientific research institutes, of which more than 50 IVD companies are listed companies in China or overseas.
About UsBCIP/NBT Concentrate 50× contains 5‑Bromo‑4‑chloro‑3‑indolyl phosphate p‑toluidine salt (BCIP) and Nitro Blue Tetrazolium chloride (NBT). It is used for the localization of alkaline phosphatase (AP)‑labeled probes in Western, Northern, Southern, and dot blot assays.
BCIP/NBT Stock Solution 50×
BCIP/NBT Concentrate 50× contains 5‑Bromo‑4‑chloro‑3‑indolyl phosphate p‑toluidine salt (BCIP) and Nitro Blue Tetrazolium chloride (NBT). It is used for the localization of alkaline phosphatase (AP)‑labeled probes in Western, Northern, Southern, and dot blot assays. BCIP serves as the substrate for AP. After dephosphorylation, the oxidized product forms a dark blue indigo dye. NBT acts as an oxidizer and also provides a dark blue chromogen, enhancing color intensity and increasing detection sensitivity.
Art no: BCIC
Package size: 10mL, 50mL, 200L, 1L, bulk
Appearance:Yellow to light brown liquid
Shelf life: 3 years
Storage: 2-8℃
Application:
Western blot、Northern blot、Microarray、ELISpot etc.
Instruction:
Preparation of 1× BCIP/NBT Working Solution: Add 200 µL of BCIP/NBT Stock Solution 50× to 10 mL of BCIP/NBT Buffer or BCIP/NBT Buffer‑DIG that has been equilibrated to room temperature. Mix well before use.
For non‑digoxigenin detection systems: Use BCIP/NBT Buffer (0.1 M Tris‑HCl, pH 9.5, containing 0.1 M NaCl and 0.05 M MgCl₂).
For digoxigenin detection systems: Use BCIP/NBT Buffer‑DIG (0.1 M Tris‑HCl, pH 9.5, containing 0.1 M NaCl, without MgCl₂). The presence of MgCl₂ may cause speckled background on the membrane after detection.
Chromogenic Reaction Procedure (Western Blot):
1.Wash: After alkaline phosphatase (AP) conjugate incubation, wash the membrane 3 times with TBST washing buffer.
2.Chromogenic Reaction: Add 1 mL substrate and incubate at 20–37 °C until bands appear dark blue.
3.Stop: Wash 2–3 times with 1.5 mL deionized water to terminate the reaction before background color develops.
4.Detection: Analyze bands by visual inspection or by imaging to quantify gray‑scale values.
For research use only or for further use in the production of detection kits. Not for human or animal use!

Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send Request B1501, Meixun Science Park,
No.19, Jinxiu Zhonglu ,
Pingshan.

