Office Location
Shenzhen, China
Production Address
B1501, Meixun Science Park,
No.19, Jinxiu Zhonglu ,
Pingshan.
Durastab Medtec LTD has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Until now, Durastab has provided products or services to more than 600 companies and 500 scientific research institutes, of which more than 50 IVD companies are listed companies in China or overseas.
About UsBCIP/NBT One Component Membrane Substrate plus produces an dark purple signal and is suitable for immunohistochemistry (IHC), as well as applications such as Western blot and microarray.
Art no: BCIS
Package size: 100mL, 1L, 5L, 10L, bulk
pH value: 9.8±0.3
Shelf life: 3 years
Storage: 2-8℃
Application:
Western blot、Immunohistochemistry、Northern blot、Microarray、ELISpot etc.
BCIP/NBT One Component Membrane Substrate plus
Durastab™ series of BCIP/NBT membrane/precipitation substrates feature low background, high stability, sensitivity, and minimal batch-to-batch variation. The series provides blue and purple signal systems, along with BCIP/NBT substrates dedicated for immunohistochemistry (IHC). BCIP/NBT One Component Membrane Substrate plus produces an dark purple signal and is suitable for immunohistochemistry (IHC), as well as applications such as Western blot and microarray.
Art no: BCIS
Package size: 100mL, 1L, 5L, 10L, bulk
pH value: 9.8±0.3
Shelf life: 3 years
Storage: 2-8℃
Application:
Western blot、Immunohistochemistry、Northern blot、Microarray、ELISpot etc.
Instruction:
Durastab™ BCIP/NBT One Component Membrane Substrate plus is ready-to-use and should be equilibrated to the reaction temperature before application.
Chromogenic Reaction Procedure (Western Blot):
1. Wash: After alkaline phosphatase (AP) conjugate incubation, wash the membrane 3 times with TBST washing buffer.
2. Chromogenic Reaction: Add 1 mL substrate and incubate at 20–37 °C until bands appear dark purple.
3. Stop: Wash 2–3 times with 1.5 mL deionized water to terminate the reaction before background color develops.
4. Detection: Analyze bands by visual inspection or by imaging to quantify grayscale values.
For research use only or for further use in the production of detection kits. Not for human or animal use!

Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send Request B1501, Meixun Science Park,
No.19, Jinxiu Zhonglu ,
Pingshan.

