Office Location
Shenzhen, China
Production Address
B1501, Meixun Science Park,
No.19, Jinxiu Zhonglu ,
Pingshan.
Durastab Medtec LTD has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Until now, Durastab has provided products or services to more than 600 companies and 500 scientific research institutes, of which more than 50 IVD companies are listed companies in China or overseas.
About UsHRP Microwell Enhanced ECL Plus is an exceptionally sensitive enhanced chemiluminescent substrate for horseradish peroxidase (HRP), offering higher sensitivity than the standard Enhanced ECL formulation. It is designed for the quantitative or qualitative detection of HRP in free-solution formats such as microwells, reaction tubes, and microfluidic systems.
HRP Microwell Enhanced ECL Plus
HRP Microwell Enhanced ECL Plus is an exceptionally sensitive enhanced chemiluminescent substrate for horseradish peroxidase (HRP), offering higher sensitivity than the standard Enhanced ECL formulation. It is designed for the quantitative or qualitative detection of HRP in free-solution formats such as microwells, reaction tubes, and microfluidic systems.An optional diluent is available to adjust the signal intensity to match the sensitivity and dynamic range requirements of specific assay systems.
Art no: CLHP
Package size: 100mL, 1000 mL, bulk
Components:
Reagent A: Enhanced Luminol Solution
Reagent B: Stabilized Peroxide Solution
Shelf life: 3 years
Storage: 2-8℃
Application:
ELISA / Chemiluminescence: Use after 1:1 premixing
Microfluidic / Microarray: Use after 1:1 premixing
Instruction
Direct Use after Mixing:
1. Prepare the working substrate by mixing Reagent A and Reagent B at a 1:1 volume ratio. For example, combine 1 mL of Enhanced Luminol Solution (CLHE-A) with 1 mL of Stable Peroxide Solution (CLHE-B). Mix thoroughly.
2. When protected from light, the mixed solution remains stable at room temperature for several hours.
Use after Diluting Reagent A:
1. Dilute Reagent A, typically at concentrations ranging from 10% to 90%. For example, to obtain 50% Reagent A, mix 50 mL of ECL Diluent with 50 mL of Reagent A.
2. Prepare the ready-to-use substrate by mixing the diluted Reagent A (e.g., 50% CLHE-A) with Reagent B at a 1:1 volume ratio. For example, combine 1 mL of 50% CLHE-A with 1 mL of CLHE-B. Mix the substrate solution thoroughly before use. When protected from light, the mixture remains stable at room temperature for several hours.
Note: HRP Microwell Enhanced ECL Plus is highly sensitive. Careful optimization of individual assay components (antibodies, conjugates, solid phase, etc.) is essential to minimize nonspecific reactions and avoid high background.
For research use only or for further use in the production of detection kits. Not for human or animal use!

Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send Request B1501, Meixun Science Park,
No.19, Jinxiu Zhonglu ,
Pingshan.

