Copyright © 2015-2025 Durastab All rights reserved
Guangdong ICP No. 2022015551-1
Newsletter
By submitting this form,you consent to receive marketing updates from Durastab.
About Us
Durastab Medtec LTD has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Until now, Durastab has provided products or services to more than 600 companies and 500 scientific research institutes, of which more than 50 IVD companies are listed companies in China or overseas.
About UsContact Info
- B1501, Meixun Science Park, No.19, Jinxiu Zhonglu , Pingshan, Shenzhen
- +86-0755-32813268
- info@durastab.com
Flagship Product: Safer, More Stable, and More Versatile – ProCide 300 Preservative!
Newly Upgraded 3rd-Gen TMB Substrate: Enhanced Stability with Multiple Freeze-Thaw Tolerance!
New Product Launch – Bovine Serum Albumin (BSA) for Clinical Biochemistry, Immunoassays, and Molecular Detection!
Flagship Product: Safer, More Stable, and More Versatile – ProCide™ 300 Preservative!
Newly Upgraded 3rd-Gen TMB Substrate: Enhanced Stability with Multiple Freeze-Thaw Tolerance!
New Product Launch – Bovine Serum Albumin (BSA) for Clinical Biochemistry, Immunoassays, and Molecular Detection!
Signaling Systems and
Immunoassay Solutions Provider
Search
SearchLuminol
HRP Microwell Femto ECL
$332/ L (500mL+500mL)
HRP Microwell Femto ECL is an ultra-sensitive enhanced chemiluminescent substrate for horseradish peroxidase (HRP), intended for the quantitative or qualitative detection of HRP in freesolution formats such as microwells, reaction tubes, and microfluidic systems. Specifically formulated for optimal protein detection performance, Femto ECL is well-suited for assays requiring detection at the lower end of the linear range in chemiluminescent ELISA applications.
HRP Microwell Femto ECL
HRP Microwell Femto ECL is an ultra‑sensitive enhanced chemiluminescent substrate for horseradish peroxidase (HRP), intended for the quantitative or qualitative detection of HRP in free‑solution formats such as microwells, reaction tubes, and microfluidic systems. The substrate incorporates a unique enhancer that delivers superior kinetic light output and exceptionally high signal intensity. Specifically formulated for optimal protein detection performance, Femto ECL is well‑suited for assays requiring detection at the lower end of the linear range in chemiluminescent ELISA applications.
Art no: ECLF
Package size: 100mL, 1000 mL, bulk
Components:
Reagent A: Enhanced Luminol Solution
Reagent B: Stabilized Peroxide Solution
Shelf life: 3 years
Storage: 2-8℃
Application:
ELISA / Chemiluminescence: Use after 1:1 premixing
Microfluidic / Microarray: Use after 1:1 premixing
Characteristics:
1. Instant Luminescence: Generates a strong signal immediately at room temperature.
2. Excellent Low‑End Linearity: Enables reliable detection of low‑abundance proteins with high signal‑to‑noise ratio and superior low‑end linearity.
3.High Sensitivity: Capable of detecting target proteins at femtogram levels in ELISA.
4. Reduced Incubation Time: High sensitivity allows shorter ELISA incubation periods.
5.Storage Stability: Stable for up to 6 months at room temperature; working solution remains stable for 8 hours.
Instruction:
Direct Use after Mixing:
1.Prepare the working substrate by mixing Reagent A and Reagent B at a 1:1 volume ratio. For example, combine 1 mL of Enhanced Luminol Solution with 1 mL of Stable Peroxide Solution and mix thoroughly.
2.The mixture remains stable at room temperature for several hours when protected from light.
Instruction:
1. Perform standard ELISA procedures including plate coating, blocking, and washing as required.
Mix Substrate A and Substrate B at a 1:1 ratio. The prepared working solution remains stable for approximately 8 hours at room temperature.
2. Add 100–150 μL of substrate working solution to each well. Optionally, use a microplate mixer to blend the liquid in the wells for 30–60 seconds.
3. Measure relative light units (RLU) using a luminometer within 1–5 minutes after adding the substrate. Prolonged incubation (e.g., >20 minutes) may result in reduced signal intensity.
Note:
1. For optimal performance, avoid prolonged exposure to strong light. The working solution should be stored protected from light. Brief exposure to typical laboratory lighting will not compromise the solution.
2. HRP Microwell Femto ECL is highly sensitive. Careful optimization of individual assay components (antibodies, conjugates, solid phase, etc.) is essential to minimize nonspecific reactions and avoid high background.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Your All-Around Partner
- 360°
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send Request