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Durastab Medtec LTD has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Until now, Durastab has provided products or services to more than 600 companies and 500 scientific research institutes, of which more than 50 IVD companies are listed companies in China or overseas.
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Flagship Product: Safer, More Stable, and More Versatile – ProCide 300 Preservative!
Newly Upgraded 3rd-Gen TMB Substrate: Enhanced Stability with Multiple Freeze-Thaw Tolerance!
New Product Launch – Bovine Serum Albumin (BSA) for Clinical Biochemistry, Immunoassays, and Molecular Detection!
Flagship Product: Safer, More Stable, and More Versatile – ProCide™ 300 Preservative!
Newly Upgraded 3rd-Gen TMB Substrate: Enhanced Stability with Multiple Freeze-Thaw Tolerance!
New Product Launch – Bovine Serum Albumin (BSA) for Clinical Biochemistry, Immunoassays, and Molecular Detection!
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pNPP One Component ELISA Substrate
$175/ L
pNPP One Component ELISA Substrate has standard sensitivity, low background, and reliable stability within the product series, and is suitable for assays with a chromogenic reaction time of less than 30 minutes.
Art no: PNST
Package size:100mL, 1L, 5L, 10L, bulk
pH value: 9.6±0.4
Shelf life:2 years
Storage: 2-8℃
Application: ELISA、biochemical reagent
pNPP One Component ELISA Substrate
Durastab™ series pNPP substrate features low background, high stability, selectable sensitivity, and minimal lot-to-lot variation, providing an excellent signal response system suitable for the development and production of detection kits. pNPP One Component ELISA Substrate has standard sensitivity, low background, and reliable stability within the product series, and is suitable for assays with a chromogenic reaction time of less than 30 minutes.
Art no: PNST
Package size:100mL, 1L, 5L, 10L, bulk
pH value: 9.6±0.4
Shelf life:2 years
Storage: 2-8℃
Application: ELISA、biochemical reagent
Instruction:
Durastab™ pNPP One Component ELISA Substrate is readytouse and should be equilibrated to the reaction temperature before application.
ELISA Chromogenic Reaction Procedure：
1. Wash: After AP conjugate incubation, wash the plate 3 times with PBST/TBST washing buffer (containing surfactant).
2. Chromogenic reaction: Add 100 μL substrate and incubate at 20–37 °C for the appropriate time ,color is yellow;
3. Stop: Add 100 μL pNPP Stop Solution;
4. Read: Measure the absorbance at 405 nm within 30 minutes,
Kinetic measurement
1. Wash: After AP conjugate incubation, wash the plate 3 times with TBST washing buffer (containing surfactant).
2. Chromogenic reaction: Add 100 μL substrate and incubate at 20–37 °C.
Read: Measure the absorbance at 405 nm at set intervals (e.g., every 1 minute).
For research use only or for further use in the production of detection kits. Not for human or animal use!
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