Durastab Medtech Co., Ltd. has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
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Durablot™ Protein-free Blocker contains no protein components, avoids cross-reaction between proteins, and is used for saturating and blocking polystyrene microplates or other solid surfaces, and can also be used as a diluent for both primary and secondary antibodies in Western blotting experiments, resulting in lower background signals.
Protein-free Blocker
Durablot™ Protein-free Blocker contains no protein components, avoids cross-reaction between proteins, and is used for saturating and blocking polystyrene microplates or other solid surfaces, and can also be used as a diluent for both primary and secondary antibodies in Western blotting experiments, resulting in lower background signals.
Art no: PFBK
Package size: 100mL, 1L, 5L, 10L, bulk
Buffer: MOPS
pH value: 7.0±0.5
Protein: None
Shelf life: 3 years
Storage: 2-8℃
Application:
Western blot：Used to seal the blot membrane, and to dilute the antibodies.
Instruction:
Durablot™ Protein-free Blocker should be equilibrated to the reaction temperature and mixed well before use.
Western blot membrane blocking (Example):
1. Protein transfer and fixation;
2. Blocking: Add an appropriate amount of Protein-free Blocker and incubate at 20-37℃ for 15-90 minutes; overnight blocking may be better.
3. Detection: Proceed with subsequent experimental steps as required.
It is recommended to dilute primary and secondary antibodies with Protein-free Blocker for lower background.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Q1: How do I choose a blocking buffer for ELISA?
A: Start from the assay matrix: BSA-based (BSAB) for standard systems, casein-based (CABS, 5%/10% concentrates) for strong background reduction on nitrocellulose and plates, Ultra for demanding low-background assays, and protein-free (PFBS, PFBK) when foreign proteins must be excluded.
Q2: What is the difference between active and passive blockers?
A: Passive blockers occupy free binding sites on the solid phase, while active blockers specifically neutralize interfering antibodies such as HAMA, rheumatoid factor and heterophilic antibodies in patient samples.
Q3: What is the difference between GelatiNSBlock-P and GelatiNSBlock-F?
A: They are gelatin-based blockers from different source materials (P and F), offering different background profiles; screen both if standard BSA/casein blockers leave residual non-specific signal.
Q4: How are the 5× and 10× casein blockers used?
A: Dilute the concentrate with your assay buffer to 1× before use; the concentrated format reduces shipping and storage volume for kit manufacturing.
Q5: Can blockers be used in Western blotting as well as ELISA?
A: Yes - casein and gelatin-based blockers are widely used on nitrocellulose and PVDF membranes, while the ELISA-focused blockers address microplate surfaces.
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send RequestDurastab Medtech (Durastab IVD) is an IVD raw materials manufacturer based in Shenzhen, China, supplying ELISA and CLIA substrates, enzyme stabilizers, blocking buffers and reagent preservatives to in vitro diagnostic manufacturers worldwide.
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