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Durastab Medtec LTD has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Until now, Durastab has provided products or services to more than 600 companies and 500 scientific research institutes, of which more than 50 IVD companies are listed companies in China or overseas.
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Flagship Product: Safer, More Stable, and More Versatile – ProCide 300 Preservative!
Newly Upgraded 3rd-Gen TMB Substrate: Enhanced Stability with Multiple Freeze-Thaw Tolerance!
New Product Launch – Bovine Serum Albumin (BSA) for Clinical Biochemistry, Immunoassays, and Molecular Detection!
Flagship Product: Safer, More Stable, and More Versatile – ProCide™ 300 Preservative!
Newly Upgraded 3rd-Gen TMB Substrate: Enhanced Stability with Multiple Freeze-Thaw Tolerance!
New Product Launch – Bovine Serum Albumin (BSA) for Clinical Biochemistry, Immunoassays, and Molecular Detection!
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TMB Board Range One Component ELISA Substrate
$155/ L
TMB Board Range One Component ELISA Substrate has a relatively wide dynamic range of detection, and a low background and stability, which is suitable for detection experiments with room temperature color development time of 10-30 minutes or 37℃.
Art no: TMBR
Package size: 100mL, 1L, 5L, 10L, bulk
pH value: 3.6±0.4
Shelf life: 4 year
Storage: 2-8℃
Application: ELISA
TMB Board Range One Component ELISA Substrate
Durastab™ TMB ELISA Substrate series features low background, high stability, minimal lot-to-lot variation, and options for different sensitivity levels.It provides an excellent signal response system suitable for the development and production of ELISA kits. TMB Board Range One Component ELISA Substrate features a broad detection dynamic range, along with low background and reliable stability, which is suitable for chromogenic reactions performed at room temperature for 10–30 minutes or at 37 °C.
Art no: TMBR
Package size:100mL, 1L, 5L, 10L, bulk
pH value: 3.6±0.4
Shelf life: 4 year
Storage: 2-8℃
Application: ELISA
Instruction:
Durastab™ TMB Board Range One Component ELISA Substrate should be equilibrated to room temperature before use.
ELISA Chromogenic Reaction Procedure：
Measurement at 450 nm:
1. Wash: After HRP conjugate incubation, wash the plate 3 times with PBST/TBST washing buffer (containing surfactant).
2. Chromogenic reaction: Add 100 μL substrate and incubate at 20–37 °C for the appropriate time, color is blue;
3. Stop: Add 100 μL TMB Stop Solution 450, the color will change yellow;
4. Read: Measure the absorbance at 450 nm within 30 minutes,
Measurement at 650 nm:
1. Wash: After HRP conjugate incubation, wash the plate 3 times with PBST/TBST washing buffer (containing surfactant).
2. Chromogenic reaction: Add 100 μL substrate and incubate at 20–37 °C for the appropriate time, color is blue;
3. Stop: Add 100 μL TMB Stop Solution 650, the color remains blue ;
4. Read: Measure the absorbance at 650 nm within 30 minutes,
Kinetic measurement at 650 nm:
1. Wash: After HRP conjugate incubation, wash the plate 3 times with PBST/TBST washing buffer (containing surfactant).
2. Chromogenic reaction: Add 100 μL substrate and incubate at 20–37 °C.
3. Read: Measure the absorbance at 650 nm at set intervals (e.g., every 1 minute).
For research use only or for further use in the production of detection kits. Not for human or animal use!
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