Durastab Medtech Co., Ltd. has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
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TMB Blue One Component Membrane Substrate (TMMB) is a precipitating TMB substrate for membrane, blot and chip-based detection. It produces a dark blue precipitate and is suitable for applications such as Western blot and microarray analysis. Art no: TMMB Package size: 100mL, 1L, 5L, 10L, bulk pH value: 4.5±0.5 Shelf life: 3 years Storage: 2-8℃ Application: Immunoblot, Western blot, Northern blot, Microarray, ELISpot etc.
Durastab™ series of membrane/ precipitation substrates features low background, high stability, sensitivity, and minimal batch-to-batch variation. The series provides blue, purple, and dark green signal systems for flexible selection in kit development and production. TMB Blue One Component Membrane Substrate produces a dark blue precipitate and is suitable for applications such as Western blot and microarray analysis.
Typical applications: Western blot, dot blot and membrane-based detection; the chip formulations extend the series to microarray and biosensor surfaces.
Instruction:
Durastab™ TMB Blue One Component Membrane Substrate is ready‑to‑use and should be equilibrated to the reaction temperature before use.
Chromogenic Reaction Procedure (Western Blot):
1. Wash：After HRP conjugate incubation, wash the membrane 3 times with PBST/TBST washing buffer;
2.Chromogenic Reaction: Add 1 mL substrate and incubate at 20–37 °C until bands appear dark blue;
3. Stop: Stop the reaction by washing 2–3 times with 1.5 mL deionized water before background develops;
4. Detection: Analyze bands by visual inspection or by imaging to quantify gray‑scale values.
Related products: TMB Enhanced One Component Membrane Substrate, TMB One Component Membrane Substrate, TMB Purple One Component Chip Substrate (TMCP), HRP Stabilizer, ProCide 300
Store at 2-8 °C protected from light. Do not freeze.
Aliquot into smaller working volumes upon receipt and avoid repeated opening of the container to reduce the risk of contamination.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Q1: What is the difference between TMB membrane substrates and TMB chip substrates?
A: Membrane formulations (TMMS, TMEN, TMMG, TMMB) are tuned for Western and dot blotting on nitrocellulose and PVDF, while chip formulations (TMEC, TMCG, TMCB, TMCP) deposit a tighter precipitate designed for microarray spots and biosensor surfaces.
Q2: Why are blue, green and purple versions available?
A: The precipitating substrates leave permanent, water-insoluble spots in different colors, so multiplex blots and arrays can distinguish several targets on one membrane without a chemiluminescence imager.
Q3: How do I stop the color development on a membrane?
A: Rinse the membrane with deionized water once the desired spot intensity is reached. The precipitated product is stable after drying and can be documented by scanner or camera.
Q4: Are these substrates supplied ready to use?
A: Yes. All membrane and chip formulations are one-component, HRP-triggered precipitating TMB substrates - no mixing step is required before use.
Q5: Can I switch from another precipitating TMB substrate to TMB Blue One Component Membrane Substrate?
A: Yes for standard membrane and chip workflows. TMB Blue One Component Membrane Substrate is a one-component, HRP-triggered precipitating formulation that develops permanent, water-insoluble spots on nitrocellulose, PVDF and array surfaces; stop with deionized water as usual and document by scanner or camera. For multiplex color layouts, pair it with the other color versions of the series.
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send RequestDurastab Medtech (Durastab IVD) is an IVD raw materials manufacturer based in Shenzhen, China, supplying ELISA and CLIA substrates, enzyme stabilizers, blocking buffers and reagent preservatives to in vitro diagnostic manufacturers worldwide.
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