Durastab Medtech Co., Ltd. has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
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DuraSure™ 5% Casein is a chemically modified, high-purity casein product commonly used as a blocking agent in immunoassay systems. Compared to BSA, gelatin, or skimmed milk powder, casein is generally more effective in reducing non-specific background signals. This product can be applied in chemiluminescence, enzyme-linked immunosorbent assays (ELISA), western blotting, lateral flow assays, and other detection methods.
5% Casein
DuraSure™ 5% Casein is a chemically modified, high-purity casein product commonly used as a blocking agent in immunoassay systems. Compared to BSA, gelatin, or skimmed milk powder, casein is generally more effective in reducing non-specific background signals. This product can be applied in chemiluminescence, enzyme-linked immunosorbent assays (ELISA), western blotting, lateral flow assays, and other detection methods. It can be used to block the surfaces of microspheres, polystyrene microplates, nitrocellulose membranes, or other solid supports, or formulated into a liquid blocking solution to achieve a higher signal-to-noise ratio and enhance the sensitivity of immunoassays.
Art no: CASE
Package size: 100mL, 1L, 5L, 10L, bulk
Buffer: None
pH value: 7.2±0.2
Protein: casein
Shelf life: 3years
Storage: 2-8℃
Application:
Western blot / ELISA/ Chemiluminescence/ Microarray
Instruction:
1. This product is supplied at 5% (w/v). It may be diluted with TBS (20 mM Tris, 150 mM NaCl, pH 7.2) before use.
For ELISA or Western blot blocking: a working concentration of 0.25–2.5% (w/v) is recommended.
If the blocked surface is to be dried directly, please use DuraBlock™ Casein Blocking Solution.
2. For microsphere blocking, a final concentration of 0.2–1% (w/v) in the blocking system is recommended.
3. For the preparation of dilution buffers, a concentration of 0.1–0.5% (w/v) is suggested.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Store at 2-8 °C.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Q1: How do I choose a blocking buffer for ELISA?
A: Start from the assay matrix: BSA-based (BSAB) for standard systems, casein-based (CABS, 5%/10% concentrates) for strong background reduction on nitrocellulose and plates, Ultra for demanding low-background assays, and protein-free (PFBS, PFBK) when foreign proteins must be excluded.
Q2: What is the difference between active and passive blockers?
A: Passive blockers occupy free binding sites on the solid phase, while active blockers specifically neutralize interfering antibodies such as HAMA, rheumatoid factor and heterophilic antibodies in patient samples.
Q3: What is the difference between GelatiNSBlock-P and GelatiNSBlock-F?
A: They are gelatin-based blockers from different source materials (P and F), offering different background profiles; screen both if standard BSA/casein blockers leave residual non-specific signal.
Q4: How are the 5× and 10× casein blockers used?
A: Dilute the concentrate with your assay buffer to 1× before use; the concentrated format reduces shipping and storage volume for kit manufacturing.
Q5: Can blockers be used in Western blotting as well as ELISA?
A: Yes - casein and gelatin-based blockers are widely used on nitrocellulose and PVDF membranes, while the ELISA-focused blockers address microplate surfaces.
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send RequestDurastab Medtech (Durastab IVD) is an IVD raw materials manufacturer based in Shenzhen, China, supplying ELISA and CLIA substrates, enzyme stabilizers, blocking buffers and reagent preservatives to in vitro diagnostic manufacturers worldwide.
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