Durastab Medtech Co., Ltd. has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
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SearchGelatiNSBlock‑F 10% is a high‑purity porcine‑skin gelatin solution, offering excellent blocking and stabilizing properties while enhancing the signal‑to‑noise ratio, sensitivity, and specificity of the reaction system. This product can be used to prepare various reaction buffers, such as sample diluents, wash buffers, and antibody diluents. It is also suitable for lyophilization protection of sensitive proteins.
GelatiNSBlock-F 10%
GelatiNSBlock‑F 10% is a high‑purity porcine‑skin gelatin solution, offering excellent blocking and stabilizing properties while enhancing the signal‑to‑noise ratio, sensitivity, and specificity of the reaction system. This product can be used to prepare various reaction buffers, such as sample diluents, wash buffers, and antibody diluents. It is also suitable for lyophilization protection of sensitive proteins. Free of blood‑derived components, GelatiNSBlock‑P 10% serves as an effective alternative to serum albumin.
Art no: GEBF
Package size: 100mL, 1L, bulk
pH value: 6.5±0.5
Protein Content: 10±0.5%
Shelf life: 3 year
Storage: 2-8℃
Application:
Western blot / ELISA/ Chemiluminescence/ Microarray/ etc.
Instruction:
Preparation of Blocking/Dilution/Working Solution: Take the required amount of GelatiNSBlock‑F 10% and add it to the desired solution. Adjust to the intended pH before use or store at 2–8 °C. Adding Tween‑20 or another surfactant at a final concentration of 0.1%–0.5% may further enhance blocking performance.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Q1: How do I choose a blocking buffer for ELISA?
A: Start from the assay matrix: BSA-based (BSAB) for standard systems, casein-based (CABS, 5%/10% concentrates) for strong background reduction on nitrocellulose and plates, Ultra for demanding low-background assays, and protein-free (PFBS, PFBK) when foreign proteins must be excluded.
Q2: What is the difference between active and passive blockers?
A: Passive blockers occupy free binding sites on the solid phase, while active blockers specifically neutralize interfering antibodies such as HAMA, rheumatoid factor and heterophilic antibodies in patient samples.
Q3: What is the difference between GelatiNSBlock-P and GelatiNSBlock-F?
A: They are gelatin-based blockers from different source materials (P and F), offering different background profiles; screen both if standard BSA/casein blockers leave residual non-specific signal.
Q4: How are the 5× and 10× casein blockers used?
A: Dilute the concentrate with your assay buffer to 1× before use; the concentrated format reduces shipping and storage volume for kit manufacturing.
Q5: Can blockers be used in Western blotting as well as ELISA?
A: Yes - casein and gelatin-based blockers are widely used on nitrocellulose and PVDF membranes, while the ELISA-focused blockers address microplate surfaces.
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send RequestDurastab Medtech (Durastab IVD) is an IVD raw materials manufacturer based in Shenzhen, China, supplying ELISA and CLIA substrates, enzyme stabilizers, blocking buffers and reagent preservatives to in vitro diagnostic manufacturers worldwide.
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