Durastab Medtech Co., Ltd. has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
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AP Microwell ECL Substrate AMPPD is an ultra-sensitive chemiluminescent substrate for alkaline phosphatase (AP), containing AMPPD and an enhancer. It is suitable for quantitative or qualitative detection of AP in free-solution formats such as microwells, reaction tubes, and microfluidic systems. AMPPD provides rapid reaction kinetics, delivering accurate and reliable results in a short time.
AP Microwell ECL Substrate AMPPD
AP Microwell ECL Substrate AMPPD is an ultra-sensitive chemiluminescent substrate for alkaline phosphatase (AP), containing AMPPD and an enhancer. It is suitable for quantitative or qualitative detection of AP in free-solution formats such as microwells, reaction tubes, and microfluidic systems. AMPPD provides rapid reaction kinetics, delivering accurate and reliable results in a short time.The AMPPD molecule comprises two key structural components: a dioxetane ring that links the phenyl group to the adamantane moiety, which cleaves to emit photons; and a phosphate group, which maintains the overall molecular stability. In the presence of AP, the phosphate group is enzymatically removed, forming an unstable intermediate that decomposes to release photons. Within a defined AP concentration range, the amount of emitted light is proportional to the enzyme concentration.
Art no: CLAM
Package size: 100mL, 1000 mL, bulk
Shelf life: 1.5 years
Storage: 2-8℃ avoid light
Application: ELISA / Chemiluminescence
Instruction:
1. Equilibrate the substrate to room temperature (18–25 °C) before use.
2. Prepare samples in a clean environment.
3. It is recommended to use 100–200 μL per assay. After adding to the reaction tube and mixing evenly, complete the luminescence measurement within the specified time.
Note: An optional diluent is available to adjust the signal intensity to match the sensitivity and dynamic range requirements of specific assay systems.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Store at 2-8 °C protected from light.
Aliquot into smaller working volumes upon receipt and avoid repeated opening of the container to reduce the risk of contamination.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Q1: Which enzyme are AMPPD and APS-5 used with?
A: Both are chemiluminescent dioxetane substrates for alkaline phosphatase (AP) in CLIA and other luminescent immunoassays.
Q2: Should I choose APS-5 or AMPPD?
A: APS-5 delivers fast peak light emission with high intensity, which suits rapid automated CLIA platforms; AMPPD produces a longer-lasting glow signal that is economical for high-throughput routine testing.
Q3: Powder or ready-to-use solution?
A: The CAS-grade powders (AMPPD, APS-5) are for self-formulation, while CLAM (AMPPD solution) and CLAP (APS-5 solution) are ready-to-use formulations that remove preparation variability.
Q4: How should the substrates be stored?
A: Ready-to-use solutions: 2-8 °C protected from light. CAS-grade powders: -20 °C protected from light (APS-5: -20 to -15 °C), aliquoted to avoid repeated freeze-thaw of stock solutions.
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send RequestDurastab Medtech (Durastab IVD) is an IVD raw materials manufacturer based in Shenzhen, China, supplying ELISA and CLIA substrates, enzyme stabilizers, blocking buffers and reagent preservatives to in vitro diagnostic manufacturers worldwide.
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