Durastab Medtech Co., Ltd. has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
About Us
Search
Search
HRP Microwell Enhanced ECL is an ultra-sensitive enhanced chemiluminescent substrate for horseradish peroxidase (HRP), intended for the quantitative or qualitative detection of HRP in free-solution formats such as microwells, reaction tubes, and microfluidic systems. An optional matching diluent is provided to adjust the signal intensity to meet the sensitivity and dynamic range requirements of specific assay systems.
HRP Microwell Enhanced ECL
HRP Microwell Enhanced ECL is an ultra-sensitive enhanced chemiluminescent substrate for horseradish peroxidase (HRP), intended for the quantitative or qualitative detection of HRP in free-solution formats such as microwells, reaction tubes, and microfluidic systems. An optional matching diluent is provided to adjust the signal intensity to meet the sensitivity and dynamic range requirements of specific assay systems.
Art no: CLHE
Package size: 100mL, 1000 mL, bulk
Components:
Reagent A: Enhanced Luminol Solution
Reagent B: Stabilized Peroxide Solution
Shelf life: 1.5 years
Storage: 2-8℃
Application:
ELISA / Chemiluminescence: Use after 1:1 premixing
Microfluidic / Microarray: Use after 1:1 premixing
Instruction:
Direct Use after Mixing:
1. Prepare the working substrate by mixing Reagent A and Reagent B at a 1:1 volume ratio. For example, combine 1 mL of Enhanced Luminol Solution (CLHE-A) with 1 mL of Stable Peroxide Solution (CLHE-B). Mix thoroughly.
2. When protected from light, the mixed solution remains stable at room temperature for several hours.
Use after Diluting Reagent A:
1. Dilute Reagent A, typically at concentrations ranging from 10% to 90%. For example, to obtain 50% Reagent A, mix 50 mL of ECL Diluent with 50 mL of Reagent A.
2. Prepare the ready-to-use substrate by mixing the diluted Reagent A (e.g., 50% CLHE-A) with Reagent B at a 1:1 volume ratio. For example, combine 1 mL of 50% CLHE-A with 1 mL of CLHE-B. Mix the substrate solution thoroughly before use. When protected from light, the mixture remains stable at room temperature for several hours.
Note: HRP Microwell Enhanced ECL is highly sensitive. Careful optimization of individual assay components (antibodies, conjugates, solid phase, etc.) is essential to minimize nonspecific reactions and avoid high background.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Store at 2-8 °C protected from light.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Q1: What are HRP microwell ECL substrates used for?
A: They are chemiluminescent HRP substrates for automated microplate CLIA platforms, covering immunoassay steps from routine testing to ultra-low abundance detection.
Q2: How do Pico, Enhanced, Plus and Femto versions differ?
A: They form a sensitivity ladder: Pico for routine assays, Enhanced for stronger signals, Plus for enhanced kinetics with extended window, and Femto for the lowest detection limits on automated CLIA systems.
Q3: Is the formulation compatible with high-throughput production?
A: Yes. Clean-room filling, tight lot-to-lot consistency and single-batch production up to 1,950 L support commercial CLIA kit manufacturing.
Q4: How should the substrates be stored?
A: Store at 2-8 °C protected from light; equilibrate to platform temperature before running.
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send RequestDurastab Medtech (Durastab IVD) is an IVD raw materials manufacturer based in Shenzhen, China, supplying ELISA and CLIA substrates, enzyme stabilizers, blocking buffers and reagent preservatives to in vitro diagnostic manufacturers worldwide.
By submitting this form,you consent to receive marketing updates from Durastab Medtech (Durastab IVD) .
