Durastab Medtech Co., Ltd. has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
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TMB Stop Solution 650 is used to terminate the color development reaction of TMB substrates. Upon termination, the reaction product remains blue and exhibits stable OD values, making it suitable for absorbance reading at 650 nm. This product does not contain SDS, thereby preventing post termination color changes, turbidity, or precipitation, and avoiding gradual decreases in OD values after stopping. As a result, it ensures more accurate detection outcomes.
TMB Stop Solution 650
TMB Stop Solution 650 is used to terminate the color development reaction of TMB substrates. Upon termination, the reaction product remains blue and exhibits stable OD values, making it suitable for absorbance reading at 650 nm. This product does not contain SDS, thereby preventing post‑termination color changes, turbidity, or precipitation, and avoiding gradual decreases in OD values after stopping. As a result, it ensures more accurate detection outcomes.
Typical applications: stopping TMB or pNPP color development for stable endpoint reading at 450 nm, 650 nm or 405 nm.
Instruction:
ELISA stopping step：
1. Chromogenic Reaction: Add 100 μL of TMB substrate solution to each well. Incubate for an appropriate period until the solution gradually turns blue.
2. Stop：Add 100 μL of TMB Stop Solution 450 to each well. Upon termination, the solution remains unchanged .
3. detection：Measure the absorbance at OD650 or OD650/492 using a microplate reader.
Related products:
TMB Broad Range One Component ELISA Substrate
TMB One Component ELISA Substrate
For research use only or for further use in the production of detection kits. Not for human or animal use!
Q1: Which stop solution matches my substrate?
A: TMB Stop Solution 450 for acid-stopped TMB endpoint reads at 450 nm, TMB Stop Solution 650 for protocols keeping the blue product, and pNPP Stop Solution 405 for AP/pNPP assays read at 405 nm.
Q2: Are the stop solutions safe to handle?
A: The formulations are designed to be non-corrosive and SDS-free, making them safer for kit production lines than concentrated mineral acids; follow the SDS for handling details.
Q3: Are concentrated wash and coating buffers available?
A: Yes. 10× wash buffers (PBST, TBST, PBS, TBS) and coating/sample-diluent buffers are supplied as concentrates to reduce shipping volume; dilute with purified water to 1× before use.
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send RequestDurastab Medtech (Durastab IVD) is an IVD raw materials manufacturer based in Shenzhen, China, supplying ELISA and CLIA substrates, enzyme stabilizers, blocking buffers and reagent preservatives to in vitro diagnostic manufacturers worldwide.
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