Copyright © 2015-2025 Durastab All rights reserved
Guangdong ICP No. 2022015551-1
Newsletter
By submitting this form,you consent to receive marketing updates from Durastab.
About Us
Durastab Medtec LTD has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
About UsContact Info
- B1501, Meixun Science Park, No.19, Jinxiu Zhonglu , Pingshan, Shenzhen
- +86-0755-32813268
- info@durastab.com
Flagship Product: Safer, More Stable, and More Versatile – ProCide 300 Preservative!
Newly Upgraded 3rd-Gen TMB Substrate: Enhanced Stability with Multiple Freeze-Thaw Tolerance!
New Product Launch – Bovine Serum Albumin (BSA) for Clinical Biochemistry, Immunoassays, and Molecular Detection!
Flagship Product: Safer, More Stable, and More Versatile – ProCide™ 300 Preservative!
Newly Upgraded 3rd-Gen TMB Substrate: Enhanced Stability with Multiple Freeze-Thaw Tolerance!
New Product Launch – Bovine Serum Albumin (BSA) for Clinical Biochemistry, Immunoassays, and Molecular Detection!
Signaling Systems and
Immunoassay Solutions Provider
Search
SearchApplications
How to Choose a TMB Substrate: Fast vs Slow, High Sensitivity vs Wide Range
TMB (3,3′,5,5′-tetramethylbenzidine) is the standard chromogenic substrate used with the HRP (horseradish peroxidase) detection enzyme in colorimetric ELISA and other HRP-based immunoassays. The signal-generation step of an immunoassay is critical to an accurate measurement, and choosing the right TMB substrate comes down to matching three assay-dependent properties: development speed (kinetic rate), sensitivity, and dynamic range. This guide explains how these trade off against one another and which Durastab™ formulation fits your workflow.
Durastab™ offers four one-component, ready-to-use TMB substrates for assay developers and kit manufacturers. They all feature low background, high stability and minimal lot-to-lot variation, and differ mainly in their kinetics and detection range — so you can choose the profile that most closely matches your immunoassay.
The Four Durastab™ TMB Substrates at a Glance
| Formulation | Art No. | Chromogenic Time | Signature Feature |
|---|---|---|---|
| High Sensitive | TMHS | ~10–15 min | High sensitivity, low background, reliable stability |
| One Component | TMST | ~10–20 min | Standard workhorse for routine ELISA |
| Board Range | TMBR | ~10–30 min | Broad detection dynamic range |
| Slow Plus | TMSP | ~15–30 min | Ultra-slow, the widest dynamic range in the series |
All Durastab™ TMB substrates are one-component, ready-to-use solutions — simply warm to room temperature and pipette. Because there is no separate component to premix, you eliminate mixing error, reduce handling time and avoid waste from unused A/B mixes, which helps streamline assay manufacturing.
How Fast Does Color Develop? Comparing the Kinetics
The chart shows color development (A450) over time under identical conditions. The steeper the curve, the faster the substrate reaches signal.
Figure 1 | Color development kinetics at low HRP load
The same comparison under a higher HRP load (4 ng/mL) is shown below — note how the faster formulations push high OD, and how High Sensitive and One Component reach the upper read limit sooner:
Figure 2 | Color development kinetics at high HRP load (4 ng/mL)
Which Substrate Fits Your Assay?
Choose High Sensitive (TMHS) if…
you measure low-abundance analytes or want to detect weak positives with minimal signal loss. Its fast ramp yields strong signal quickly — just control the stop time.
Choose One Component (TMST) if…
you run routine batch ELISAs and want a dependable, middle-of-the-road substrate with no mixing.
Choose Board Range (TMBR) if…
your standard curve spans a wide concentration range and you want accurate quantification across it. The broader linear range reduces re-runs on out-of-range samples.
Choose Slow Plus (TMSP) if…
you need an ultra-slow substrate with the widest dynamic range — ideal for automation, high-throughput runs, or applications where a forgiving timing window matters.
Fast vs Slow: What Changes with Development Speed?
Fast substrates (High Sensitive) save time and push signal higher, but color keeps climbing, so timing the stop is more critical. Slow substrates (Slow Plus) trade a little speed for a wide, forgiving endpoint and steadier automation behavior.
High Sensitivity vs Wide Dynamic Range: The Trade-Off
High sensitivity improves detection at low concentrations. Wide dynamic range extends the linear portion of the standard curve. Simply put: sensitivity helps you detect less; dynamic range helps you quantify more accurately across a spectrum.
Three Tips Before You Decide
Keep conditions consistent when comparing — same HRP concentration, substrate volume and temperature.
Mind platform temperature; development is faster at 37 °C than at room temperature.
Plan the endpoint read: read at 450 nm right after stopping, or at 650 nm if reading without stopping.
Frequently Asked Questions
How do I choose a TMB substrate? Match speed, sensitivity and range to your assay. Low-concentration detection favors High Sensitive; wide-ranging samples favor Board Range or Slow Plus; routine work favors One Component.
Which is the most sensitive? High Sensitive develops signal fastest and is built for maximum sensitivity in low-analyte assays.
Do you offer a slow TMB? Yes — Slow Plus develops over roughly 15–30 min and gives a forgiving timing window.
Are all four ready-to-use? Yes. All are one-component, ready-to-use solutions stored at 2–8 °C, read at 450 nm after stopping (or 650 nm without stopping).
Need a sample or large-volume quote? Contact sales@durastab.com
Quality First
We have established a comprehensive quality management system and use stringent standards to make our products more reliable.
We can achieve excellent batch-to-batch consistency and a maximum single-batch output of 1950L, ensuring a stable supply to companies worldwide.
Our Advantages
- Strong R&D capabilities
- Strong supply capacity
- Product boasts Excellent performance and Stability
- Further reduce your costs
- We have GMP-Standard production workshops
Our Mission：Make Diagnosis more Accurate and Stable Serving the Life.
Durastab Medtech Co., Ltd.
Our Vision：To become a globally renowned manufacturer of key raw materials for diagnostic kits.
Advocating Green and environmentally friendly solutions
We are committed to developing green and environmentally friendly reagents, using environmentally friendly materials, and reducing pollution.
We advocate against using environmentally harmful components, especially VOCs such as methanol and DMF, to protect the ecosystem and create a safer working environment.
Environmentally Friendly Components: Our product line offers a variety of environmentally friendly reagents that are free of any organic solvents, toxic substances, animal-derived ingredients, BSA, or other components that may have adverse effects on the ecological or working environment.
Top Choice for Leading Companies: We have long been committed to developing green and environmentally friendly reagent products. Nevertheless, our products do not sacrifice quality, sensitivity, and accuracy, making us the preferred choice for most leading immunoassay kit manufacturers.
Your All-Around Partner
- 360°
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send Request
Leave Your Message
If you have any suggestions or question for us.Please contact us.