Durastab Medtech Co., Ltd. has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
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Durablock™ Casein Blocking Solution is an optimized blocking system based on high‑purity chemically modified casein, delivering superior blocking performance for saturating polystyrene microplates or other solid surfaces. This effectively reduces background, lowers the coefficient of variation (CV), and enhances detection sensitivity.
Casein Blocking Solution
Durablock™ Casein Blocking Solution is an optimized blocking system based on high‑purity chemically modified casein, delivering superior blocking performance for saturating polystyrene microplates or other solid surfaces. This effectively reduces background, lowers the coefficient of variation (CV), and enhances detection sensitivity. Upon incubation with the Casein Blocking Solution, unoccupied sites on the microplate or nitrocellulose membrane become saturated, preventing nonspecific binding of analytes or detection antibodies to the surface. This minimizes background and improves assay sensitivity. Based on chemically modified, high‑purity casein, this product significantly outperforms other commercial or in‑house casein solutions prepared by simple dissolution and filtration. For many assays, Casein Blocking Solution provides maximal saturation blocking and exceptionally low inter‑assay variation. The high‑purity, chemically modified casein molecules help minimize interactions between the blocking layer and the sample matrix. This results in both highly efficient blocking for more reliable detection outcomes and a reduction in cross‑reactivity while maximizing surface coverage.
Art no: CABS
Package size: 100mL, 500mL, 1L, bulk
Buffer: None
pH value: 7.2±0.4
Protein: casein
Shelf life: 3 years
Storage: 2-8℃
Application:
ELISA：Ready‑to‑use for blocking after antibody or antigen coating.
Western blotting：Ready‑to‑use for blocking after membrane coating.
Instruction:
Durablock™ Casein Blocking Solution is ready‑to‑use. Allow it to equilibrate to the reaction temperature, then mix well before application.
Microplate Blocking Procedure (Example):
1.After antigen or antibody coating;
2.Wash: Remove unbound protein by washing twice with PBS/TRIS washing buffer (without surfactant);
3.Blocking: Add an appropriate amount of Durablock™ Casein Blocking Solution and incubate at 20–37 °C for 30–60 minutes;
4.Detection: Detection: Aspirate (or blot off) the Casein Blocking Solution and proceed directly to downstream detection; alternatively, wash away excess blocking solution twice with PBS/TRIS washing buffer (without surfactant) before performing downstream detection.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Store at 2-8 °C.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Q1: How do I choose a blocking buffer for ELISA?
A: Start from the assay matrix: BSA-based (BSAB) for standard systems, casein-based (CABS, 5%/10% concentrates) for strong background reduction on nitrocellulose and plates, Ultra for demanding low-background assays, and protein-free (PFBS, PFBK) when foreign proteins must be excluded.
Q2: What is the difference between active and passive blockers?
A: Passive blockers occupy free binding sites on the solid phase, while active blockers specifically neutralize interfering antibodies such as HAMA, rheumatoid factor and heterophilic antibodies in patient samples.
Q3: What is the difference between GelatiNSBlock-P and GelatiNSBlock-F?
A: They are gelatin-based blockers from different source materials (P and F), offering different background profiles; screen both if standard BSA/casein blockers leave residual non-specific signal.
Q4: How are the 5× and 10× casein blockers used?
A: Dilute the concentrate with your assay buffer to 1× before use; the concentrated format reduces shipping and storage volume for kit manufacturing.
Q5: Can blockers be used in Western blotting as well as ELISA?
A: Yes - casein and gelatin-based blockers are widely used on nitrocellulose and PVDF membranes, while the ELISA-focused blockers address microplate surfaces.
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send RequestDurastab Medtech (Durastab IVD) is an IVD raw materials manufacturer based in Shenzhen, China, supplying ELISA and CLIA substrates, enzyme stabilizers, blocking buffers and reagent preservatives to in vitro diagnostic manufacturers worldwide.
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