Durastab Medtech Co., Ltd. has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
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Protein-free Blocking Solution contains no protein components and is free of any animal‑derived materials. It is specifically optimized for serum antibody detection (e.g., indirect antigen‑coated assays). Containing no protein, the product eliminates inter‑protein cross‑reactivity. It is designed for saturating and blocking polystyrene microplates or other solid surfaces, and is also suitable for blocking other protein‑coated surfaces, making it compatible with most detection systems.
Protein-free Blocking Solution
Protein-free Blocking Solution contains no protein components and is free of any animal‑derived materials. It is specifically optimized for serum antibody detection (e.g., indirect antigen‑coated assays). This solution saturates unoccupied sites to prevent nonspecific binding of serum antibodies to the solid surface, while also protecting against displacement of conventional blocking molecules by interfering substances such as antibodies present in serum or plasma samples. Additionally, it helps avoid cross‑reactions between serum proteins and conventional protein‑based blocking agents. Containing no protein, the product eliminates inter‑protein cross‑reactivity. It is designed for saturating and blocking polystyrene microplates or other solid surfaces, and is also suitable for blocking other protein‑coated surfaces, making it compatible with most detection systems.
Art no: PFBS
Package size: 100mL, 500mL, 1L, bulk
Buffer: PBS
pH value: 7.0±0.4
Protein: None
Shelf life: 3 years
Storage: 2-8℃
Application:
ELISA：Ready‑to‑use for blocking after antibody or antigen coating.
Instruction:
Durablock™ Protein-free Blocking Solution is ready‑to‑use. Allow it to equilibrate to the reaction temperature, then mix well before application.
Microplate Blocking Procedure (Example):
1.After antigen or antibody coating;
2.Wash: Remove unbound protein by washing twice with PBS/TRIS washing buffer (without surfactant);
3.Blocking: Add an appropriate amount of Durablock™ Protein-free Blocking Solution and incubate at 20–37 °C for 30–60 minutes;
4.Detection: Detection: Aspirate (or blot off) the Protein-free Blocking Solution and proceed directly to downstream detection; alternatively, wash away excess blocking solution twice with PBS/TRIS washing buffer (without surfactant) before performing downstream detection.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Store at 2-8 °C.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Q1: How do I choose a blocking buffer for ELISA?
A: Start from the assay matrix: BSA-based (BSAB) for standard systems, casein-based (CABS, 5%/10% concentrates) for strong background reduction on nitrocellulose and plates, Ultra for demanding low-background assays, and protein-free (PFBS, PFBK) when foreign proteins must be excluded.
Q2: What is the difference between active and passive blockers?
A: Passive blockers occupy free binding sites on the solid phase, while active blockers specifically neutralize interfering antibodies such as HAMA, rheumatoid factor and heterophilic antibodies in patient samples.
Q3: What is the difference between GelatiNSBlock-P and GelatiNSBlock-F?
A: They are gelatin-based blockers from different source materials (P and F), offering different background profiles; screen both if standard BSA/casein blockers leave residual non-specific signal.
Q4: How are the 5× and 10× casein blockers used?
A: Dilute the concentrate with your assay buffer to 1× before use; the concentrated format reduces shipping and storage volume for kit manufacturing.
Q5: Can blockers be used in Western blotting as well as ELISA?
A: Yes - casein and gelatin-based blockers are widely used on nitrocellulose and PVDF membranes, while the ELISA-focused blockers address microplate surfaces.
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send RequestDurastab Medtech (Durastab IVD) is an IVD raw materials manufacturer based in Shenzhen, China, supplying ELISA and CLIA substrates, enzyme stabilizers, blocking buffers and reagent preservatives to in vitro diagnostic manufacturers worldwide.
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