Durastab Medtech Co., Ltd. has always adhered to the mission of "making diagnostic reagents more stable and more accurate" since its establishment, and is committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
To date, Durastab has successfully provided products to over 600 companies and research teams at thousands of universities and scientific research institutions worldwide, among which are more than 50 listed IVD companies.
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Durablock™ Ultra Blocking Solution is a peptide‑based blocking formulation containing chemically modified peptide components. It provides highly efficient blocking for more reliable detection outcomes while maximizing surface coverage and minimizing cross‑reactivity. The use of Ultra Blocking Solution also eliminates potential interference caused by serum components such as BSA in assay analyses.
Ultra Blocking Solution
Durablock™ Ultra Blocking Solution is a peptide‑based blocking formulation containing chemically modified peptide components. It is designed for saturating polystyrene microplates or other solid surfaces. Through incubation, the unoccupied sites on the microplate or nitrocellulose membrane are saturated with Ultra Blocking Solution, which prevents nonspecific binding of analytes or detection antibodies to the surface, thereby reducing background signals. For many assays, Ultra Blocking Solution serves as a cost‑effective and efficient alternative to BSA‑based blocking solutions. It provides highly efficient blocking for more reliable detection outcomes while maximizing surface coverage and minimizing cross‑reactivity. The use of Ultra Blocking Solution also eliminates potential interference caused by serum components such as BSA in assay analyses.
Art no: UTBS
Package size: 100mL, 500mL, 1L, bulk
pH value: 7.0±0.5
Protein: BSA free
Shelf life: 3 years
Storage: 2-8℃
Application:
ELISA：Ready‑to‑use for blocking after antibody or antigen coating.
Instruction:
Durablock™ Ultra blocking solution is ready‑to‑use. Allow it to equilibrate to the reaction temperature, then mix well before application.
Microplate Blocking Procedure (Example):
1.After antigen or antibody coating;
2.Wash: Remove unbound protein by washing twice with PBS/TRIS washing buffer (without surfactant);
3.Blocking: Add an appropriate amount of Durablock™ Ultra blocking solution and incubate at 20–37 °C for 30–60 minutes;
4.Detection: Aspirate (or blot off) the Ultra blocking solution and proceed directly to downstream detection; alternatively, wash away excess blocking solution twice with PBS/TRIS washing buffer (without surfactant) before performing downstream detection.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Store at 2-8 °C.
For research use only or for further use in the production of detection kits. Not for human or animal use!
Q1: How do I choose a blocking buffer for ELISA?
A: Start from the assay matrix: BSA-based (BSAB) for standard systems, casein-based (CABS, 5%/10% concentrates) for strong background reduction on nitrocellulose and plates, Ultra for demanding low-background assays, and protein-free (PFBS, PFBK) when foreign proteins must be excluded.
Q2: What is the difference between active and passive blockers?
A: Passive blockers occupy free binding sites on the solid phase, while active blockers specifically neutralize interfering antibodies such as HAMA, rheumatoid factor and heterophilic antibodies in patient samples.
Q3: What is the difference between GelatiNSBlock-P and GelatiNSBlock-F?
A: They are gelatin-based blockers from different source materials (P and F), offering different background profiles; screen both if standard BSA/casein blockers leave residual non-specific signal.
Q4: How are the 5× and 10× casein blockers used?
A: Dilute the concentrate with your assay buffer to 1× before use; the concentrated format reduces shipping and storage volume for kit manufacturing.
Q5: Can blockers be used in Western blotting as well as ELISA?
A: Yes - casein and gelatin-based blockers are widely used on nitrocellulose and PVDF membranes, while the ELISA-focused blockers address microplate surfaces.
Committed to providing excellent ready-to-use reagent solutions to global advanced medical diagnostic companies.
Send RequestDurastab Medtech (Durastab IVD) is an IVD raw materials manufacturer based in Shenzhen, China, supplying ELISA and CLIA substrates, enzyme stabilizers, blocking buffers and reagent preservatives to in vitro diagnostic manufacturers worldwide.
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